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Critical Review-Bisulfite-treatment free DNA methylation detection

Patterns of DNA methylation are altered in cancer cells and play important roles in cancer development and progression. These altered DNA methylation marks can prove to be valuable tools for early diagnosis of cancer as well as monitoring of response to therapy. It is because of these reasons that interest in detecting DNA methylation with high sensitivity has grown over the years. However, most of the clinically useful methods for DNA methylation detection rely on the bisulfite-treatment (BT). Though BT is one of the most widely used DNA methylation detection approaches, low conversion, false reading, longer assay time etc. are some of its shortcomings. In recent years numerous bisulfite-free methods have been developed, and in this paper we have reviewed recent developments in BT-independent electrochemical and optical biosensors in DNA methylation detection. We have also highlighted the current challenges associated with these BT free detection methods. This review paper also sheds light on the current progress towards the incorporation of these electrochemical and optical readouts based bisulfite free methods into standard patient care.

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