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(3) Eugene J. H. Wee, Muhammad J. A. Shiddiky, MattTrau (2013)

‘Detecting DNA Methylation for Cancer Diagnostics and Prognostics’

in  Microfluidics, Nanotechnology and Disease Biomarkers for Personalized  Medicine Applications, Muhammad J.A. Shiddiky,Eugene J. H. Wee,  Sakandar Rauf, Matt Trau (Eds.). Nova Science Publishers, Inc., USA. ISBN: 978-1- 62808-039- 1. Chapter 6.

Publication year: 2013

Abstract

In recent years, thousands of cancer biomarkers have been discovered and described in scientific literature. The promise of personalized medicine, where diseases such as cancer are accurately diagnosed and treatments tailored specifically for individuals, is becoming a reality. Significant advances in biomarker-based research methodologies such as Next Generation Sequencing (NGS) are at the cusp of ushering in a new era of personal medicine. However, unlike the spectacular advances in research technologies for disease biomarker discovery, biomarker-based technologies that can effectively be used in the clinic (or point-of-care) to enable personalized medicine are still lacking. In this book, we feature a selection of emerging technologies which are aimed at enabling clinical applications of personalised medicine. Each of the eight chapters is written by a leading group at the intersection of microfluidics, biology, and nanotechnology. For instance, to accelerate a major bottleneck in the development of clinically useful protein diagnostics, we discuss the application of yeast-derived single chain Fragment variable (scFv) antibody-like molecules as a potential low cost alternative to traditional antibody-based diagnostics. Circulating tumour cells (CTCs) are an emerging class of cancer biomarkers and a potential resource for understanding cancer progression; we explore various strategies combining microfluidics with nanotechnology for capturing CTCs. The book includes an evaluation of some current and emerging technologies for detecting clinical DNA methylation, another potential cancer biomarker. As personalized medicine may involve tracking a patient’s response to treatment, the application of microfluidics to detect metabolites in biological fluids is also discussed. Finally, the ultimate goal of personalized medicine is targeted therapy. One promising approach is RNAi technology which uses short nucleotides to disrupt cancer pathways. In this book, nanoparticle approaches to deliver these short nucleotides are discussed.

(2) Muhammad J. A. Shiddiky, Angle A. J. Torriero (2011)

‘Electrochemical Biosensor Based on Ionic Liquids’

in Electrochemical Properties and  Applications of Ionic Liquids, M. J. A. Shiddiky & A. A. J. Torriero, (Eds.). Nova Science Publishers, Inc., USA. ISBN 978-1- 61728-942- 2. Chapter 11.

Publication year: 2011

Abstract

Since 1992, when the room temperature ionic liquids (ILs) based on the 1-alkyl-3-methylimidazolium cation were reported to provide an attractive combination of an electrochemical solvent and electrolyte, ILs have been widely used in electrodeposition, electrosynthesis, electrocatalysis, electrochemical capacitor, and lithium batteries. However, it has only been in the last few years that electrochemical biosensors based on carbon ionic liquid electrodes (CILEs) and IL-modified macrodisk electrodes have been reported. However, there are still a lot of challenges in achieving IL-based sensitive, selective, and reproducible biosensors for high speed analysis of biological and environmental compounds of interest. This review discusses the principles of operation of electrochemical biosensors based on CILEs and IL/composite-modified macrodisk electrodes. Subsequently, recent developments and major strategies for enhancing sensing performance are discussed. Key challenges and opportunities of IL-based biosensors to further development and use are considered. Emphasis is given to direct electron-transfer reaction and electrocatalysis of hemeproteins and enzyme-modified composite electrodes.

(1) Muhammad J. A. Shiddiky, Yoon-Bo Shim, (2010)

‘Microchip and Capillary Electrophoresis Using Nanoparticles’

in Microfluidic Devices in  Nanotechnology: Applications, C. S. Kumar, (Ed.). John Wiley & Sons, Inc., Weinheim, 213 – 253. ISBN 978-0- 470-59069- 0. Chapter 6.

Publication year: 2010